By using the knock-in allele Lgr5 EGFP-Ires-CreERT2, we have shown that the cell surface receptor Lgr5 (leucine-rich repeat–containing heterotrimeric guanine nucleotide–binding protein–coupled receptor 5) marks normal tissue stem cells in stomach, small intestine, colon, and hair follicles .
Lgr5-eGFP-IRES-CreERT2 and Bmi1-CreER; Rosa26-YFP mice were used to compare the basal proliferation status of Lgr5 + vs. Bmi1 + ISCs during homeostasis. We used short-term tamoxifen exposure, for induction of Cre-mediated recombination, to selectively mark Bmi1 + ISCs in vivo.
5, or 7 d showed a In sharp contrast to control SPA-Cre; Cdh1fl/+; Lgr5-eGFP-IRES-CreERT2 mice, where GFP- positive Lgr5+ cells resided at the base of the glands in both the Lgr5-EGFP-IRES-CreERT2/Rosa-LSL-tdTomato mice. To label only Lgr5 stem cells, Lgr5-EGFP-IRES-CreERT2/Rosa-LSL-tdTomato mouse was injected with. For genetic lineage tracing, Lgr5-EGFP-IRES-cre/ERT2 mice were crossed with Rosa26-tdTomato mice. To induce the expression of the reporter gene, a single We crossed Lgr5-EGFP-IRES-CreERT2 knock-in and ROSA26-tdTomato (LRT) mice, and treated them with or without 4-hydroxytamoxifen (4-OHT), to determine Aug 1, 2012 To study the behavior of Lgr5+ cells within Apc-mutant adenomas, we then crossed Lgr5EGFP-Ires-CreERT2/Apcfl/fl mice with the R26R-Confetti Exogenous IFN-γ depletes Paneth cells and modifies the expression of rapidly cycling ISC markers. Heterozygous Lgr5 Egfp Ires Creert2 Mice, supplied by The Jan 15, 2021 Organoids from Lgr5-EGFP-ires-CreERT2-TdT mice were transplanted in C57Bl6 male mice exposed to PIR. Engraftment and repopulation of (C) MGs were isolated from Lgr5-EGFP mice and analyzed by flow cytometry for iments, Lgr5-EGFP-IRES-creERT2 mice were crossed into the LifeAct-RFP. Also Known As:Lgr5-EGFP-IRES-creERT2. Heterozygous mice harbor an Lgr5- EGFP-IRES-creERT2 "knock-in" allele that both abolishes Lgr5 gene function Jun 12, 2019 (B-H) Embryonic liver organoids were generated from sorted Lgr5-GFP+ hepatoblasts isolated from E10.5-E12.5 Lgr5-EGFP-IRES-creERT2 quence of adenoma formation, we generated Lgr5-EGFP-.
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In control mice, lineage traced cells populated the entire length of the villi (Fig. 2 a) in the majority (69.9%) of the villi (Additional file 3 Lgr5-(EGFP-IRES-creERT2) Cat. NO. NM-KI-200154. Name * Company * Phone No. * Email * Research Field. Remark. Submit. Contact us Gene-editing proposal Back to top Heterozygous mice harbor an Lgr5-EGFP-IRES-creERT2 "knock-in" allele that both abolishes Lgr5 gene function and expresses EGFP and CreERT2 fusion protein. When these mice mice are bred with mice containing a loxP-flanked sequence of interest, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the floxed sequences in the Lgr5-expressing cells of the offspring.
在线客服 ; 实验动物一站式采购平台 Symbol: Lgr5 tm1(cre/ERT2)Cle: Name: leucine rich repeat containing G protein coupled receptor 5; targeted mutation 1, Hans Clevers: MGI ID: MGI:3764660: Synonyms: Lgr5-cre, Lgr5CREER, Lgr5-creERT, Lgr5-CreERT2-IRES-EGFP, Lgr5-EGFP, Lgr5 EGFP-IRES-creERT2, Lgr5-EGFP-IRES-creERT2, Lgr5 tm1(cre/ESR1)Cle cells in Lgr5-eGFP-IRES-CreERT2 mice.
Lgr5-EGFP-Ires-CreERT2;R26R-Confetti double transgenic mice were treated with vehicle (n = 8) or G007-LK (n = 5) and tamoxifen according to the two administration schemes outlined in Fig. 1d, f. In control mice, lineage traced cells populated the entire length of the villi (Fig. 2 a) in the majority (69.9%) of the villi (Additional file 3
Lgr5/GFP+ve cells were isolated using flow cytometry. 2010-01-08 · Lgr5-EGFP-ires-CreERT2 mice were injected with BrdU 2 hr prior to sacrifice in order to visualize actively cycling cells within the stomach. This revealed the presence of S-phase (i.e., cycling) cells within the Lgr5-EGFP +ve population at the gland base ( Figures 1 G and 1H; red arrows), although proliferation was generally less frequent than at the isthmus above the neck of the pyloric glands.
Lgr5-EGFP-ires-creERT2 mice,11 APCfl/fl mice,25 Axin2-lacZ mice,26 and C57B/6 wild-type mice (6–12 weeks old) were used for experiments. Lgr5-EGFP-ires-creERT2 mice were crossed with APCfl/fl mice. Cre enzyme activity was induced by intraperitoneal injections of tamoxifen (2 mg/mouse). Murine small intestines
All animals were housed in a facility with a 12‐h light/12‐h dark cycle. ApcMin and Lgr5-EGFP-ires-CreERT2 mice were obtained from The Jackson Laboratory (Bar Harbor, ME). Foxf1 and Foxf2 targeted mutants have been described elsewhere.22,23 Tg(FOXF2) was generated by pronuclear injection of a linearized BAC (RP4-668J24) spanning 120,429 base pairs of the human FOXF2 locus. tiation. We have employed the crypts from Lgr5‐EGFP‐IRES‐creERT2 reporter mouse line to grow Lgr5+ enteroids.10 In this article, we sum‐ marize the procedure of organoid culture derived from single Lgr5+ cells, and provide a simple way to examine their viability and functions. An overview of the experimental workflow is given in Figure 1.
AX10 (Standard) Animal Health Reports Facility Barrier Level Descriptions Pricing & Availability Available Live mice available in varying quantities. Lgr5 Egfp Ires Creert2, supplied by The Jackson Laboratory, used in various techniques.
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In this mouse model, GFP expression is driven from the Lgr5 locus, leading to highest GFP levels in Lgr5 + cells (GFP high). pressesLgr5-EGFPintheLgr5EGFP-Ires-CreERT2knock-inmice.The results showed that Lgr5-EGFP cells did not express the HBC markers, K14 and ICAM-1 (Fig. 1D,E). Instead, Lgr5-EGFP cells were localized immediately above the ICAM-1 or K14-positive HBCs, and displayed a distinct round morphology that was more characteristic of the GBC population Lgr5-eGFP-IRES-CreERT2 and Bmi1-CreER; Rosa26-YFP mice were treated with 12 Gy whole-body irradiation.
Adaptations to defined media components in the wake of advances in ES and iPS cell culture has led to the identification of conditions that maintained intestinal cell progenitors in culture.
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Additionally, we exposed Lgr5-EGFP-IRES-CreERT2 mice to azoxymethane/dextrane sodium sulfate (AOM/DSS), which induces inflammation-driven colon tumors. Tumors were then flow-sorted into fractions of epithelial cells that expressed high or low levels of Lgr5 and were molecularly characterized using gene expression profiling and array comparative genomic hybridization.
In control mice, lineage traced cells populated the entire length of the villi (Fig. 2 a) in the majority (69.9%) of the villi (Additional file 3 Lgr5-(EGFP-IRES-creERT2) Cat. NO. NM-KI-200154. Name * Company * Phone No. * Email * Research Field. Remark.
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Lgr5-EGFP-IRES-creERT2 "knock-in" allele that both abolishes Lgr5 (Gpr49) gene function and expresses EGFP and CreERT2 fusion protein from the Lgr5 promoter/enhancer elements. EGFP fluorescence is observed in crypt base columnar The Jackson Laboratory lgr5 egfp ires creert2 Lgr5 Egfp Ires Creert2, supplied by The Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more Lgr5-cre, Lgr5CREER, Lgr5-creERT, Lgr5-CreERT2-IRES-EGFP, Lgr5-EGFP, Lgr5 EGFP-IRES-creERT2, Lgr5-EGFP-IRES-creERT2, Lgr5 tm1(cre/ESR1)Cle: Gene: Lgr5 Location: Chr10:115450314-115587780 bp, - strand Genetic Position: Chr10, 63.51 cM In Lgr5-eGFP-IRES-CreERT2 and Bmi1-CreER; Rosa26-YFP mice, canonical Wnt signaling was potently induced by systemic administration of an adenovirus encoding Rspo1 fused to an IgG2α Fc fragment (Ad Rspo1-Fc), causing marked crypt hypertrophy and hyperproliferation. By 5 d after infection, Ad Rspo1-Fc markedly expanded Lgr5-eGFP+ The Jackson Laboratory lgr5 egfp ires creert2 Lgr5 Egfp Ires Creert2, supplied by The Jackson Laboratory, used in various techniques.
When these mice mice are bred with mice containing a loxP-flanked sequence of interest, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the floxed sequences in the Lgr5-expressing cells of the offspring.